Composite
Part:BBa_K2004011:Design
Designed by: Marco V. Lincango Y. Group: iGEM16_BIOSINT_Mexico (2016-10-18)
CDO and reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1335
Design Notes
Codon optimized for E. coli and sequences free of restrictions sites.
Source
NCBI databank. Sequences taken from E. aerogenes